Growth and electrophysiological properties of rat embryonic cardiomyocytes on hydroxyl- and carboxyl-modified surfaces

    Authors

    A. Natarajan; C. J. Chun; J. J. Hickman;P. Molnar

    Comments

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    Abbreviated Journal Title

    J. Biomater. Sci.-Polym. Ed.

    Keywords

    Cardiomyocytes; cell culture; electrophysiology; cardiac tissue; engineering; serum-free; SAM; hydroxyl; carboxyl; scaffolds; PLA; PLGA; SELF-ASSEMBLED MONOLAYERS; SERUM-FREE MEDIUM; PROTEIN ADSORPTION; DEFINED SYSTEM; CELL-ADHESION; MOLECULAR ARCHITECTURE; EXTRACELLULAR-MATRIX; DEGRADATION BEHAVIOR; POLYMER DEGRADATION; IN-VITRO; Engineering, Biomedical; Materials Science, Biomaterials; Polymer; Science

    Abstract

    Biodegradable scaffolds such as poly(lactic acid) (PLA), poly(lactic-co-glycolic acid) (PLGA) or poly(glycolic acid) (PGA) are commonly used materials in tissue engineering. The chemical composition of these scaffolds changes during degradation which provides a differential environment for the seeded cells. In this study we have developed a simple and relatively high-throughput method in order to test the physiological effects of this varying chemical environment on rat embryonic cardiac myocytes. In order to model the different degradation stages of the scaffold, glass coverslips were functionalized with 11-mercaptoundecanoic acid (MUA) and 11-mercapto-1-undecanol (MUL) as carboxyl-and hydroxyl-groups presenting surfaces, and with trimethoxysilylpropyldiethylenetriamine (DETA) and (3-aminopropyl) triethoxysilane (APTES) as controls. Embryonic cardiac myocytes formed beating islands on all tested surfaces, but the number of attached cells and beating patches was significantly lower on MUL compared to any of the other functionalized surfaces. Moreover, whole-cell patch-clamp experiments showed that the average length of action potentials generated by the beating-cardiac myocytes were significantly longer on MUL compared to the other surfaces. Our results, using our simple test system, are in basic agreement with earlier observations that utilized a complex 3D biodegradable scaffold. Thus, surface functionalization with self-assembled monolayers combined with histological/physiological testing could be a relatively high throughput method for biocompatibility studies and for the optimization of the material/tissue interface in tissue engineering.

    Journal Title

    Journal of Biomaterials Science-Polymer Edition

    Volume

    19

    Issue/Number

    10

    Publication Date

    1-1-2008

    Document Type

    Article

    Language

    English

    First Page

    1319

    Last Page

    1331

    WOS Identifier

    WOS:000262267500005

    ISSN

    0920-5063

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