Two-Photon Fluorescence Lysosomal Bioimaging with a Micelle-Encapsulated Fluorescent Probe

    Authors

    C. D. Andrade; C. O. Yanez; M. A. Qaddoura; X. H. Wang; C. L. Arnett; S. A. Coombs; J. Yu; R. Bassiouni; M. V. Bondar;K. D. Belfield

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    Abbreviated Journal Title

    J. Fluoresc.

    Keywords

    Fluorescent dyes; Pluronic; Multiphoton absorption; Bioimaging; Fluorescence microscopy; Lysosomes; STIMULATED-EMISSION-DEPLETION; OPTICAL-DATA STORAGE; FLUORENE; DERIVATIVES; CROSS-SECTIONS; BECLIN 1; MICROSCOPY; AUTOPHAGY; RESOLUTION; TRANSPORT; NM; Biochemical Research Methods; Chemistry, Analytical; Chemistry, Physical

    Abstract

    We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic (R) F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.

    Journal Title

    Journal of Fluorescence

    Volume

    21

    Issue/Number

    3

    Publication Date

    1-1-2011

    Document Type

    Article

    Language

    English

    First Page

    1223

    Last Page

    1230

    WOS Identifier

    WOS:000291999400050

    ISSN

    1053-0509

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