Two-Photon Fluorescence Vascular Bioimaging with New Bioconjugate Probes Selective toward the Vascular Endothelial Growth Factor Receptor 2

    Authors

    C. D. Andrade; C. O. Yanez; H. Y. Ahn; T. Urakami; M. V. Bondar; M. Komatsu;K. D. Belfied

    Comments

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    Abbreviated Journal Title

    Bioconjugate Chem.

    Keywords

    PHOTOACOUSTIC MICROSCOPY; TUMOR-GROWTH; ANGIOGENESIS; MICROVASCULATURE; NORMALIZATION; METASTASIS; EXCITATION; TARGETS; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Multidisciplinary; Chemistry, Organic

    Abstract

    We report the synthesis and characterization of two amine reactive fluorescent dyes with efficient two-photon absorption (2PA) Properties and high-fluorescence quantum yields. Bioconjugation of these dyes with the DC-101 antibody proved to be useful for selectively imaging the vascular endothelial growth factor receptor 2 (VEGFR-2) in cells expressing this receptor in vitro and in "whole" mounted excised tumors (ex vivo) by two-photon fluorescence microscopy (2PFM). The penetration depths reached within the tumors by 2PFM was over 800 mu m. In addition, the concentration of dye required for incubation of these bioconjugates was in the picomolar domain, the probes possessed very good photostability. and the 2PFM setup did not require any additional means of increasing the collection efficiencies of fluorescent photons to achieve the relatively deep tissue imaging that was realized, due, in large part, to the favorable photophysical properties of the new probes.

    Journal Title

    Bioconjugate Chemistry

    Volume

    22

    Issue/Number

    10

    Publication Date

    1-1-2011

    Document Type

    Article

    Language

    English

    First Page

    2060

    Last Page

    2071

    WOS Identifier

    WOS:000295915100021

    ISSN

    1043-1802

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