Title

Two-Photon Fluorescence Vascular Bioimaging with New Bioconjugate Probes Selective toward the Vascular Endothelial Growth Factor Receptor 2

Authors

Authors

C. D. Andrade; C. O. Yanez; H. Y. Ahn; T. Urakami; M. V. Bondar; M. Komatsu;K. D. Belfied

Comments

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Abbreviated Journal Title

Bioconjugate Chem.

Keywords

PHOTOACOUSTIC MICROSCOPY; TUMOR-GROWTH; ANGIOGENESIS; MICROVASCULATURE; NORMALIZATION; METASTASIS; EXCITATION; TARGETS; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Multidisciplinary; Chemistry, Organic

Abstract

We report the synthesis and characterization of two amine reactive fluorescent dyes with efficient two-photon absorption (2PA) Properties and high-fluorescence quantum yields. Bioconjugation of these dyes with the DC-101 antibody proved to be useful for selectively imaging the vascular endothelial growth factor receptor 2 (VEGFR-2) in cells expressing this receptor in vitro and in "whole" mounted excised tumors (ex vivo) by two-photon fluorescence microscopy (2PFM). The penetration depths reached within the tumors by 2PFM was over 800 mu m. In addition, the concentration of dye required for incubation of these bioconjugates was in the picomolar domain, the probes possessed very good photostability. and the 2PFM setup did not require any additional means of increasing the collection efficiencies of fluorescent photons to achieve the relatively deep tissue imaging that was realized, due, in large part, to the favorable photophysical properties of the new probes.

Journal Title

Bioconjugate Chemistry

Volume

22

Issue/Number

10

Publication Date

1-1-2011

Document Type

Article

Language

English

First Page

2060

Last Page

2071

WOS Identifier

WOS:000295915100021

ISSN

1043-1802

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