Two-Photon STED Spectral Determination for a New V-Shaped Organic Fluorescent Probe with Efficient Two-Photon Absorption

    Authors

    K. D. Belfield; M. V. Bondar; A. R. Morales; L. A. Padilha; O. V. Przhonska;X. H. Wang

    Comments

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    Abbreviated Journal Title

    ChemPhysChem

    Keywords

    absorption; fluorescence; fluorene derivatives; laser scanning; fluorescence microscopy; two-photon stimulated emission depletion; STIMULATED-EMISSION-DEPLETION; OPTICAL-DATA STORAGE; FLUORENE; DERIVATIVES; MICROSCOPY; LASER; ANISOTROPY; SOLVENT; FLUOROPHORES; FIELD; WAVE; Chemistry, Physical; Physics, Atomic, Molecular & Chemical

    Abstract

    Two-photon stimulated emission depletion (STED) cross sections were determined over a broad spectral range for a novel two-photon absorbing organic molecule, representing the first such report. The synthesis, comprehensive linear photophysical, two-photon absorption (2PA), and stimulated emission properties of a new fluorene-based compound, (E)-2-{3-[2-(7(diphenylamino)-9,9-diethyl-9H-fluoren-2-yl)vinyl]-5-methyl-4-oxocyclohexa-2,5-dienylidene} malononitrile (1), are presented. Linear spectral parameters, including excitation anisotropy and fluorescence lifetimes, were obtained over a broad range of organic solvents at room temperature. The degenerate two-photon absorption (2PA) spectrum of 1 was determined with a combination of the direct open-aperture Z-scan and relative two-photon-induced fluorescence methods using 1 kHz femtosecond excitation. The maximum value of the 2PA cross section similar to 1700 GM was observed in the main, long wavelength, one-photon absorption band. One-and two-photon stimulated emission spectra of 1 were obtained over a broad spectral range using a femtosecond pump-probe technique, resulting in relatively high two-photon stimulated emission depletion cross sections (similar to 1200 GM). A potential application of 1 in bioimaging was demonstrated through one-and two-photon fluorescence microscopy images of HCT 116 cells incubated with micelle-encapsulated dye.

    Journal Title

    Chemphyschem

    Volume

    12

    Issue/Number

    15

    Publication Date

    1-1-2011

    Document Type

    Article

    Language

    English

    First Page

    2755

    Last Page

    2762

    WOS Identifier

    WOS:000297017600013

    ISSN

    1439-4235

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