Title
Integrin-Targeting Block Copolymer Probes for Two-Photon Fluorescence Bioimaging
Abbreviated Journal Title
Biomacromolecules
Keywords
ABSORPTION CROSS-SECTIONS; GENERAL SYNTHETIC ROUTE; TUMOR VASCULATURE; CELL-ADHESION; QUANTUM DOTS; RGD PEPTIDES; PHASE-I; MICROSCOPY; CHROMOPHORES; ANGIOGENESIS; Biochemistry & Molecular Biology; Chemistry, Organic; Polymer Science
Abstract
Targeted molecular imaging with two-photon fluorescence microscopy (2PFM) is a powerful technique for chemical biology and, potentially, for noninvasive diagnosis and treatment of a number of diseases. The synthesis, photophysical studies, and bioimaging are reported for a versatile norbornene-based block copolymer multifunctional scaffold containing biocompatible (PEG), two-photon fluorescent dyes (fluorenyl) and targeting (cyclic-RGD peptide) moieties. The two bioconjugates, containing two different fluorenyl dyes and cRGDfK covalently attached to the polymer probe, formed a spherical micelle and self-assembled structure in water, for which size was analyzed by transmission electron microscopy (TEM) and dynamic light scattering (DLS). Cell viability and 2PFM imaging of human epithelial U87MG cell lines that overexpress alpha(v)beta(3) integrin was performed via incubation with the new probes, along with negative control studies using MCF-7 breast cancer cells and blocking experiments. 2PFM microscopy confirmed the high selectivity of the biocompatible probe in the integrin-rich area in the U87MF cells while blocking as well as negative control MCF-7 experiments confirmed the integrin-targeting ability of the new probes.
Journal Title
Biomacromolecules
Volume
12
Issue/Number
2
Publication Date
1-1-2011
Document Type
Article
DOI Link
Language
English
First Page
441
Last Page
449
WOS Identifier
ISSN
1525-7797
Recommended Citation
"Integrin-Targeting Block Copolymer Probes for Two-Photon Fluorescence Bioimaging" (2011). Faculty Bibliography 2010s. 1104.
https://stars.library.ucf.edu/facultybib2010/1104
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