Title
In vitro cell-toxicity screening as an alternative animal model for coral toxicology: effects of heat stress, sulfide, rotenone, cyanide, and cuprous oxide on cell viability and mitochondrial function
Abbreviated Journal Title
Ecotoxicology
Keywords
Cell isolation; Coral; Cyanide; Cytotoxicity; Cuprous oxide; Sulfide; NONYL-ACRIDINE-ORANGE; SPECTROPHOTOMETRIC DETERMINATION; COLORIMETRIC; ASSAY; HYDROGEN-SULFIDE; BLEACHING EVENT; REEF HEALTH; SMOKING; POLLUTION; FUTURE; DEATH; Ecology; Environmental Sciences; Toxicology
Abstract
The logistics involved in obtaining and maintaining large numbers of corals hampers research on the toxicological effects of environmental contaminants for this ecologically and economically important taxon. A method for creating and culturing single-cell suspensions of viable coral cells was developed. Cell segregation/separation was based on specific cell densities and resulting cell cultures were viable for at least 2 mos. Low-density cells lacking symbiotic zooxanthallae and rich in mitochondria were isolated and cultured for toxicity studies. Cells were exposed to differing degrees or concentrations of heat stress, rotenone, cyanide, sulfide, and cuprous oxide. Cells were assayed for mitochondrial membrane potential using the fluorescent probe, JC-9, and for overall viability using the MTT/formazan spectrophotometric viability assay. Significant differences were observed between controls and treatments and the efficacy of this method was validated; only 2 cm(2) of tissue was required for a seven-point concentration-exposure series.
Journal Title
Ecotoxicology
Volume
19
Issue/Number
1
Publication Date
1-1-2010
Document Type
Article
Language
English
First Page
171
Last Page
184
WOS Identifier
ISSN
0963-9292
Recommended Citation
"In vitro cell-toxicity screening as an alternative animal model for coral toxicology: effects of heat stress, sulfide, rotenone, cyanide, and cuprous oxide on cell viability and mitochondrial function" (2010). Faculty Bibliography 2010s. 120.
https://stars.library.ucf.edu/facultybib2010/120
Comments
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