Title

ES cells overexpressing microRNA-1 attenuate apoptosis in the injured myocardium

Authors

Authors

C. Glass;D. K. Singla

Comments

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Abbreviated Journal Title

Mol. Cell. Biochem.

Keywords

Apoptosis; Heart; microRNA; Akt; PTEN; EMBRYONIC STEM-CELLS; INHIBIT APOPTOSIS; PROGENITOR CELLS; GROWTH-FACTORS; H9C2 CELLS; DIFFERENTIATION; INFARCTION; HEART; EXPRESSION; REPAIR; Cell Biology

Abstract

MicroRNAs (miRs) are small, single-stranded, noncoding RNA's involved in post-transcriptional negative gene regulation. Recent investigations have underscored the integral role of miRs in various biological processes including innate immunity, cell-cycle regulation, metabolism, differentiation, and cell death. In the present study, we overexpressed miR-1, a muscle-specific miR, in embryonic stem cells (miR-1-ES cells), transplanted them into the infarcted myocardium, and evaluated their impact on cardiac apoptosis and function. We provide evidence demonstrating reduced apoptosis following transplantation of miR-1-ES cells 4 weeks post-myocardial infarction as compared to respective controls assessed by TUNEL staining and a capsase-3 activity assay. Moreover, we show significant elevation in p-Akt levels and diminished PTEN levels in hearts transplanted with miR-1-ES cells as determined by enzyme-linked immunoassays. Finally, using echocardiography, we reveal mice receiving miR-1-ES cell transplantation post-myocardial infarction had significantly improved fractional shortening and ejection fraction compared with respective controls. Our data suggest transplanted miR-1-ES cells inhibit apoptosis, mediated through the PTEN/Akt pathway, leading to improved cardiac function in the infarcted myocardium.

Journal Title

Molecular and Cellular Biochemistry

Volume

357

Issue/Number

1-2

Publication Date

1-1-2011

Document Type

Article

Language

English

First Page

135

Last Page

141

WOS Identifier

WOS:000295941500015

ISSN

0300-8177

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