Title

A Single Molecular Beacon Probe Is Sufficient for the Analysis of Multiple Nucleic Acid Sequences

Authors

Authors

Y. V. Gerasimova; A. Hayson; J. Ballantyne;D. M. Kolpashchikov

Comments

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Abbreviated Journal Title

ChemBioChem

Keywords

biosensors; DNA recognition; molecular beacons; nucleic acids; NUCLEOTIDE POLYMORPHISMS; HUMAN GENOME; RNA DETECTION; LIVING CELLS; DNA; HYBRIDIZATION; FLUORESCENCE; NANOTECHNOLOGY; IDENTIFICATION; DEOXYRIBOZYME; Biochemistry & Molecular Biology; Chemistry, Medicinal

Abstract

Molecular beacon (MB) probes are dual-labeled hairpin-shaped oligodeoxyribonucleotides that are extensively used for real-time detection of specific RNA/DNA analytes. In the MB probe, the loop fragment is complementary to the analyte: therefore, a unique probe is required for the analysis of each new analyte sequence. The conjugation of an oligonucleotide with two dyes and subsequent purification procedures add to the cost of MB probes, thus reducing their application in multiplex formats. Here we demonstrate how one MB probe can be used for the analysis of an arbitrary nucleic acid The approach fakes advantage of two oligonucleotide adaptor strands, each of which contains a fragment complementary to the analyte and a fragment complementary to an MB probe The presence of the analyte leads to association of MB probe and the two DNA strands in quadripartite complex The MB probe fluorescently reports the formation of this complex In this design, the MB does not bind the analyte directly; therefore, the MB sequence is independent of the analyte. In this study one universal MB probe was used to genotype three human polymorphic sites. This approach promises to reduce the cost of multiplex real-time assays and improve the accuracy of single-nucleotide polymorphism genotyping

Journal Title

Chembiochem

Volume

11

Issue/Number

12

Publication Date

1-1-2010

Document Type

Article

Language

English

First Page

1762

Last Page

1768

WOS Identifier

WOS:000281538500019

ISSN

1439-4227

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