Analysis of co-eluted isomers of high-molecular weight polycyclic aromatic hydrocarbons in high performance liquid chromatography fractions via solid-phase nanoextraction and time-resolved Shpol'skii spectroscopy

    Authors

    W. B. Wilson;A. D. Campiglia

    Comments

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    Abbreviated Journal Title

    J. Chromatogr. A

    Keywords

    High-molecular weight polycyclic aromatic hydrocarbons; Solid-phase; nanoextraction; Shpol'skii spectroscopy; Gold nanoparticles; Laser-excited time-resolved Shpol'skii spectroscopy; MULTIDIMENSIONAL FLUORESCENCE SPECTROSCOPY; IONIZATION; MASS-SPECTROMETRY; DRINKING-WATER SAMPLES; 15 PRIORITY POLLUTANTS; ENVIRONMENTAL-SAMPLES; DIBENZOPYRENE ISOMERS; NANO-EXTRACTION; HPLC; FRACTIONS; FACILE METHOD; CARBON-BLACK; Biochemical Research Methods; Chemistry, Analytical

    Abstract

    We present an accurate method for the determination of isomers of high-molecular weight polycyclic aromatic hydrocarbons co-eluted in HPLC fractions. The feasibility of this approach is demonstrated with two isomers of molecular weight 302 with identical mass fragmentation patterns, namely dibenzo[a,i]pyrene and naphtho[2,3-a]pyrene. Qualitative and quantitative analysis is carried out via laser-excited time-resolved Shpol'skii spectroscopy at liquid helium temperature. Unambiguous identification of co-eluted isomers is based on their characteristic 4.2 K line-narrowed spectra in n-octane as well as their fluorescence lifetimes. Pre-concentration of HPLC fractions prior to spectroscopic analysis is performed with the aid of gold nanoparticles via an environmentally friendly procedure. In addition to the two co-eluted isomers, the analytical figures of merit of the entire procedure were evaluated with dibenzo[a,l]pyrene, dibenzo[a,h]pyrene and dibenzo[a,e]pyrene. The analytical recoveries from drinking water samples varied between 98.2 +/- 5.5 (dibenzo[a,l]pyrene) and 102.7 +/- 3.2% (dibenzo[a,i]pyrene). The limits of detection ranged from 51.1 ng L(-1) (naphtho[2,3-a]pyrene) to 154 ng L(-1) (dibenzo[a,e]pyrene). The excellent analytical figures of merit associated to its HPLC compatibility makes this approach an attractive alternative for the analysis of co-eluted isomers with identical mass spectra. (C) 2011 Elsevier B.V. All rights reserved.

    Journal Title

    Journal of Chromatography A

    Volume

    1218

    Issue/Number

    39

    Publication Date

    1-1-2011

    Document Type

    Article

    Language

    English

    First Page

    6922

    Last Page

    6929

    WOS Identifier

    WOS:000295241500010

    ISSN

    0021-9673

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