Two-Photon Absorption and Time-Resolved Stimulated Emission Depletion Spectroscopy of a New Fluorenyl Derivative

    Authors

    K. D. Belfield; M. V. Bondar; A. R. Morales; X. L. Yue; G. Luchita; O. V. Przhonska;O. D. Kachkovsky

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    Abbreviated Journal Title

    ChemPhysChem

    Keywords

    bioimaging; fluorene derivatives; time-resolved fluorescence; spectroscopy; two-photon absorption; two-photon stimulated emission; depletion; OPTICAL-DATA STORAGE; STED MICROSCOPY; FLUORESCENT-PROBE; NONLINEARITIES; FLUOROPHORES; ANISOTROPY; MOLECULES; LIFETIME; DESIGN; DYES; Chemistry, Physical; Physics, Atomic, Molecular & Chemical

    Abstract

    The synthesis, comprehensive linear photophysical characterization, two-photon absorption (2PA), steady-state and time-resolved stimulated emission depletion properties of a new fluorene derivative, (E)-1-(2-(di-p-tolylamino)-9,9-diethyl-9H-fluoren-7-yl)-3-(thiophen-2-yl)prop-2-en-1-one (1), are reported. The primary linear spectral properties, including excitation anisotropy, fluorescence lifetimes, and photostability, were investigated in a number of aprotic solvents at room temperature. The degenerate 2PA spectra of 1 were obtained with open-aperture Z-scan and two-photon induced fluorescence methods, using a 1 kHz femtosecond laser system, and maximum 2PA cross-sections of similar to 400600 GM were obtained. The nature of the electronic absorption processes in 1 was investigated by DFT-based quantum chemical methods implemented in the Gaussian 09 program. The one- and two-photon stimulated emission spectra of 1 were measured over a broad spectral range using a femtosecond pumpprobe-based fluorescence quenching technique, while a new methodology for time-resolved fluorescence emission spectroscopy is proposed. An effective application of 1 in fluorescence bioimaging was demonstrated by means of one- and two-photon fluorescence microscopy images of HCT 116 cells containing dye encapsulated micelles.

    Journal Title

    Chemphyschem

    Volume

    13

    Issue/Number

    15

    Publication Date

    1-1-2012

    Document Type

    Article

    Language

    English

    First Page

    3481

    Last Page

    3491

    WOS Identifier

    WOS:000309887300010

    ISSN

    1439-4235

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