Title

Small Molecule Induction of Human Umbilical Stem Cells into Myelin Basic Protein Positive Oligodendrocytes in a Defined Three-Dimensional Environment

Authors

Authors

H. Davis; X. F. Guo; S. Lambert; M. Stancescu;J. J. Hickman

Comments

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Abbreviated Journal Title

ACS Chem. Neurosci.

Keywords

Immunochemistry; in vitro test; mesenchymal stem cell; neural cell; surface modification; three-dimensional; RAT SPINAL-CORD; CENTRAL-NERVOUS-SYSTEM; GLIAL-CELLS; ADULT-RAT; IN-VITRO; HIPPOCAMPAL-NEURONS; CARDIAC MYOCYTES; DIFFERENTIATION; CULTURE; PROGENITORS; Biochemistry & Molecular Biology; Chemistry, Medicinal; Neurosciences

Abstract

Umbilical cord stem cells would be a favorable alternative to embryonic stem cells for therapeutic applications. In this study, human multipotent progenitor cells (MLPCs) from umbilical cord were differentiated into oligodendrocytes by being exposed to a range of micro-environmental chemical and physical cues. Chemical cues were represented by a novel defined differentiation medium containing the neurotransmitter norepinephrine (NE). Under traditional two-dimensional conditions, the MLPCs differentiated into oligodendrocyte precursors but did not progress further. However, in a three-dimensional environment, the MLPCs differentiated into. committed oligodendrocytes that expressed myelin basic protein. The apparent method of interaction of NE in stimulating the differentiation process was shown to occur through the adenergic pathway, while all prior differentiation methods have used other routes. This novel method of obtaining functional human oligodendrocytes from MLPCs would eliminate many of the difficulties associated with their differentiation from embryonic stem cells.

Journal Title

Acs Chemical Neuroscience

Volume

3

Issue/Number

1

Publication Date

1-1-2012

Document Type

Article

Language

English

First Page

31

Last Page

39

WOS Identifier

WOS:000299353800006

ISSN

1948-7193

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