Small Molecule Induction of Human Umbilical Stem Cells into Myelin Basic Protein Positive Oligodendrocytes in a Defined Three-Dimensional Environment

    Authors

    H. Davis; X. F. Guo; S. Lambert; M. Stancescu;J. J. Hickman

    Comments

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    Abbreviated Journal Title

    ACS Chem. Neurosci.

    Keywords

    Immunochemistry; in vitro test; mesenchymal stem cell; neural cell; surface modification; three-dimensional; RAT SPINAL-CORD; CENTRAL-NERVOUS-SYSTEM; GLIAL-CELLS; ADULT-RAT; IN-VITRO; HIPPOCAMPAL-NEURONS; CARDIAC MYOCYTES; DIFFERENTIATION; CULTURE; PROGENITORS; Biochemistry & Molecular Biology; Chemistry, Medicinal; Neurosciences

    Abstract

    Umbilical cord stem cells would be a favorable alternative to embryonic stem cells for therapeutic applications. In this study, human multipotent progenitor cells (MLPCs) from umbilical cord were differentiated into oligodendrocytes by being exposed to a range of micro-environmental chemical and physical cues. Chemical cues were represented by a novel defined differentiation medium containing the neurotransmitter norepinephrine (NE). Under traditional two-dimensional conditions, the MLPCs differentiated into oligodendrocyte precursors but did not progress further. However, in a three-dimensional environment, the MLPCs differentiated into. committed oligodendrocytes that expressed myelin basic protein. The apparent method of interaction of NE in stimulating the differentiation process was shown to occur through the adenergic pathway, while all prior differentiation methods have used other routes. This novel method of obtaining functional human oligodendrocytes from MLPCs would eliminate many of the difficulties associated with their differentiation from embryonic stem cells.

    Journal Title

    Acs Chemical Neuroscience

    Volume

    3

    Issue/Number

    1

    Publication Date

    1-1-2012

    Document Type

    Article

    Language

    English

    First Page

    31

    Last Page

    39

    WOS Identifier

    WOS:000299353800006

    ISSN

    1948-7193

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