Protein complexes/aggregates as potential cancer biomarkers revealed by a nanoparticle aggregation immunoassay

    Authors

    Q. Huo

    Comments

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    Abbreviated Journal Title

    Colloid Surf. B-Biointerfaces

    Keywords

    Protein complex/aggregate; Cancer biomarker; Gold nanoparticle; Immunoassay; Dynamic light scattering; DYNAMIC LIGHT-SCATTERING; CIRCULATING TUMOR-CELLS; MARKERS; PROBES; Biophysics; Chemistry, Physical; Materials Science, Biomaterials

    Abstract

    Protein-protein interactions and protein complex/aggregate formation play an essential role in almost all biological functions and activities. Through a nanoparticle aggregation immunoassay, we discovered that some proteins are substantially more complexed/aggregated in cancer tissues than normal tissues. This study examined four biomarkers proteins, CA125, CEA (carcinoembryonic antigen), CA19-9 and PAP (prostatic acid phosphatase) in ovarian, colon and prostate tissue lysates. The most exciting results were observed from the PAP assay of prostate tissues: prostate cancer can be clearly distinguished from normal prostate and prostate with benign conditions such as BPH (benign prostate hyperplasia) based on the complex/aggregation level of PAP in prostate tissue lysates. The complex/aggregate level of a protein can be potential biomarkers for cancer detection and diagnosis. (C) 2010 Elsevier B.V. All rights reserved.

    Journal Title

    Colloids and Surfaces B-Biointerfaces

    Volume

    78

    Issue/Number

    2

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    259

    Last Page

    265

    WOS Identifier

    WOS:000278167100016

    ISSN

    0927-7765

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