Organic electrochemical transistor based immunosensor for prostate specific antigen (PSA) detection using gold nanoparticles for signal amplification

    Authors

    D. J. Kim; N. E. Lee; J. S. Park; I. J. Park; J. G. Kim;H. J. Cho

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    Abbreviated Journal Title

    Biosens. Bioelectron.

    Keywords

    Prostate cancer; Conducting polymer; Organic electronics; Gold; nanoparticle; Immunosensor; SURFACE-PLASMON RESONANCE; BIOLOGICAL SENSORS; LABELS; IMMUNOASSAY; MICROELECTRODES; BIOSENSORS; ANTIBODY; PH; Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology

    Abstract

    We demonstrated a highly sensitive organic electrochemical transistor (OECT) based immunosensor with a low detection limit for prostate specific antigen/alpha 1-antichymotrypsin (PSA-ACT) complex. The poly(styrenesulfonate) doped poly(3,4-ethylenedioxythiophene) (PEDOT:PSS) based OECT with secondary antibody conjugated gold nanoparticles (AuNPs) provided a detection limit of the PSA-ACT complex as low as 1 pg/ml, as well as improved sensitivity and a dynamic range, due to the role of AuNPs in the signal amplification. The sensor performances were particularly improved in the lower concentration range where the detection is clinically important for the preoperative diagnosis and screening of prostate cancer. This result shows that the OECT-based immunosensor can be used as a transducer platform acceptable to the point-of-care (POC) diagnostic systems and demonstrates adaptability of organic electronics to clinical applications. (C) 2010 Elsevier B.V. All rights reserved.

    Journal Title

    Biosensors & Bioelectronics

    Volume

    25

    Issue/Number

    11

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    2477

    Last Page

    2482

    WOS Identifier

    WOS:000279095200015

    ISSN

    0956-5663

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