A Nanochannel Array-Based Electrochemical Device for Quantitative Label-free DNA Analysis

    Authors

    S. J. Li; J. Li; K. Wang; C. Wang; J. J. Xu; H. Y. Chen; X. H. Xia;Q. Huo

    Comments

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    Abbreviated Journal Title

    ACS Nano

    Keywords

    nanochannel array; porous anodic alumina membrane; label free sensor; morpholino; DNA; electrochemical analysis; GOLD NANOTUBULE MEMBRANES; POROUS ALUMINA MEMBRANES; SINGLE; NANOCHANNELS; ANODIC ALUMINA; TRANSPORT; SELECTIVITY; HYBRIDIZATION; BIOSENSORS; NANOPORES; PROBES; Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience &; Nanotechnology; Materials Science, Multidisciplinary

    Abstract

    A strategy for label free oligonucleotide (DNA) analysis has been proposed,by measuring the DNA Morpholino hybridization,hindered diffusion,flux of probe ion Fe(CN)(6)(3-) through nanochannels of a porous anodic alumina (PAA) membrane The flux of Fe(CN)(6)(3-) passing through the PAA nanochannels is recorded using an Au film electrochemical detector sputtered at the end of the nanochannels Hybridization of the end tethered morpholino in the nanochannel with DNA forms a negatively charged DNA-morpholino complex, which hinders the diffusion of Fe(CN)(6)(3-) through the nanochannels and results in a decreased flux This flux is strongly dependent and target DNA concentration, which indicates a synergetic effect on ionic strength, nanochannel aperture, and target DNA concentration, which indicates a synergetic effect of steric and electrostatic repulsion effects in the confined nanochannels Further comparison of the probe flux with different charge passing through the nanochannels confirms that the electrostatic effect between the probe ions and DNA dominates the hindered diffusion process Under optimal conditions, the present nanochannel array based DNA biosensor give a detection limit of 0 1 nM

    Journal Title

    Acs Nano

    Volume

    4

    Issue/Number

    11

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    6417

    Last Page

    6424

    WOS Identifier

    WOS:000284438000016

    ISSN

    1936-0851

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