A Differential Fluorescent Receptor for Nucleic Acid Analysis

    Authors

    H. N. Bengtson;D. M. Kolpashchikov

    Comments

    Authors: contact us about adding a copy of your work at [email protected]

    Abbreviated Journal Title

    ChemBioChem

    Keywords

    differential receptors; dumbbell molecular beacon; principal component; analysis; SNP analysis; RESISTANT MYCOBACTERIUM-TUBERCULOSIS; DOUBLE-CROSSOVER MOLECULES; TIME; PCR ASSAY; RIFAMPIN RESISTANCE; XPERT MTB/RIF; SENSOR ARRAYS; EXTRAPULMONARY TUBERCULOSIS; SPECIES IDENTIFICATION; PATTERN-RECOGNITION; REACTIVE ARRAYS; Biochemistry & Molecular Biology; Chemistry, Medicinal

    Abstract

    Differential receptors use an array of sensors to recognize analytes. Each sensor in the array can recognize not one, but several analytes with different rates, so a single analyte triggers a response of several sensors in the array. The receptor thus produces a pattern of signals that is unique for each analyte, thereby enabling identification of a specific analyte by producing a fingerprint pattern. We applied this approach for the analysis of DNA sequences of Mycobacterium tuberculosis strains that differ by single nucleotide substitutions in the 81-bp hot-spot region that imparts rifampin resistance. The technology takes advantage of the new multicomponent, selfassembling sensor, which produces a fluorescent signal in the presence of specific DNA sequences. A differential fluorescent receptor (DFR) contained an array of three such sensors and differentiated at least eight DNA sequences. The approach requires only one molecular-beacon-like fluorescent reporter, which can be used by all three sensors. The DFR developed in this study represents a cost-efficient alternative to molecular diagnostic technologies that use fluorescent hybridization probes.

    Journal Title

    Chembiochem

    Volume

    15

    Issue/Number

    2

    Publication Date

    1-1-2014

    Document Type

    Article

    Language

    English

    First Page

    228

    Last Page

    231

    WOS Identifier

    WOS:000329877400007

    ISSN

    1439-4227

    Share

    COinS
     

    Accessibility Statement

    This item was created or digitized prior to April 24, 2027, or is a reproduction of legacy media created before that date. It is preserved in its original, unmodified state specifically for research, reference, or historical recordkeeping. In accordance with the ADA Title II Final Rule, the University Libraries provides accessible versions of archival materials upon request. To request an accommodation for this item, please submit an accessibility request form.