A Differential Fluorescent Receptor for Nucleic Acid Analysis

    Authors

    H. N. Bengtson;D. M. Kolpashchikov

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    ChemBioChem

    Keywords

    differential receptors; dumbbell molecular beacon; principal component; analysis; SNP analysis; RESISTANT MYCOBACTERIUM-TUBERCULOSIS; DOUBLE-CROSSOVER MOLECULES; TIME; PCR ASSAY; RIFAMPIN RESISTANCE; XPERT MTB/RIF; SENSOR ARRAYS; EXTRAPULMONARY TUBERCULOSIS; SPECIES IDENTIFICATION; PATTERN-RECOGNITION; REACTIVE ARRAYS; Biochemistry & Molecular Biology; Chemistry, Medicinal

    Abstract

    Differential receptors use an array of sensors to recognize analytes. Each sensor in the array can recognize not one, but several analytes with different rates, so a single analyte triggers a response of several sensors in the array. The receptor thus produces a pattern of signals that is unique for each analyte, thereby enabling identification of a specific analyte by producing a fingerprint pattern. We applied this approach for the analysis of DNA sequences of Mycobacterium tuberculosis strains that differ by single nucleotide substitutions in the 81-bp hot-spot region that imparts rifampin resistance. The technology takes advantage of the new multicomponent, selfassembling sensor, which produces a fluorescent signal in the presence of specific DNA sequences. A differential fluorescent receptor (DFR) contained an array of three such sensors and differentiated at least eight DNA sequences. The approach requires only one molecular-beacon-like fluorescent reporter, which can be used by all three sensors. The DFR developed in this study represents a cost-efficient alternative to molecular diagnostic technologies that use fluorescent hybridization probes.

    Journal Title

    Chembiochem

    Volume

    15

    Issue/Number

    2

    Publication Date

    1-1-2014

    Document Type

    Article

    Language

    English

    First Page

    228

    Last Page

    231

    WOS Identifier

    WOS:000329877400007

    ISSN

    1439-4227

    Share

    COinS