Characterization of liposomes and silica nanoparticles using resistive pulse method

    Authors

    Y. Ruthevich; Y. Q. Lin; A. Wearne; A. Ordonez; O. Lupan;L. Chow

    Comments

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    Abbreviated Journal Title

    Colloid Surf. A-Physicochem. Eng. Asp.

    Keywords

    Nanoparticles; Electrophoresis; Nanopores; Liposomes; Translocations; Nanopipette; COULTER-COUNTER; PARTICLES; DNA; TRANSPORT; NANOPORES; SIZE; Chemistry, Physical

    Abstract

    The ability to precisely count inorganic and organic nanoparticles and to measure their size distribution plays a major role in various applications such as drug delivery, nanoparticles counting, and many others. Here we employ a simple resistive pulse method that allows translocations, counting, and measuring size and velocity distribution of silica nanoparticles and liposomes with diameters from 50 nm to 250 nm. This technique is based on the Coulter counter technique but has nanometer size pores. It was found that ionic current drops when nanoparticles enter the nanopore of a pulled micropipette, producing a clear translocation signal. Pulled borosilicate micropipettes with opening 50-350 nm were used as the detecting instrument. This method provides a direct, fast and cost-effective way to characterize inorganic and organic nanoparticles in a solution. (C) 2014 Elsevier B.V. All rights reserved.

    Journal Title

    Colloids and Surfaces a-Physicochemical and Engineering Aspects

    Volume

    448

    Publication Date

    1-1-2014

    Document Type

    Article

    Language

    English

    First Page

    9

    Last Page

    15

    WOS Identifier

    WOS:000334141100002

    ISSN

    0927-7757

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