Design of a Multi-Dopamine-Modified Polymer Ligand Optimally Suited for Interfacing Magnetic Nanoparticles with Biological Systems

    Authors

    W. T. Wang; X. Ji; H. B. Na; M. Safi; A. Smith; G. Palui; J. M. Perez;H. Mattoussi

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    Langmuir

    Keywords

    IRON-OXIDE NANOPARTICLES; QUANTUM DOTS; HYDROPHOBIC NANOCRYSTALS; FE3O4; NANOPARTICLES; AMPHIPHILIC POLYMER; CHEMICAL DESIGN; CONTRAST AGENTS; CANCER; WATER; MRI; Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, ; Multidisciplinary

    Abstract

    We have designed a set of multifunctional and multicoordinating polymer ligands that are optimally suited for surface functionalizing iron oxide and potentially other magnetic nanoparticles (NPs) and promoting their integration into biological systems. The amphiphilic polymers are prepared by coupling (via nucleophilic addition) several amine-terminated dopamine anchoring groups, poly(ethylene glycol) moieties, and reactive groups onto a poly(isobutylene-alt-maleic anhydride) (PIMA) chain. This design greatly benefits from the highly efficient and reagent-free one-step reaction of maleic anhydride groups with amine-containing molecules. The availability of several dopamine groups in the same ligand greatly enhances the ligand affinity, via multiple coordination, to the magnetic NPs, while the hydrophilic and reactive groups promote colloidal stability in buffer media and allow subsequent conjugation with target biomolecules. Iron oxide nanoparticles ligand exchanged with these polymer ligands have a compact hydrodynamic size and exhibit enhanced long-term colloidal stability over the pH range of 4-12 and in the presence of excess electrolytes. Nanoparticles ligated with terminally reactive polymers have been easily coupled to target dyes and tested in live cell imaging with no measurable cytotoxicity. Finally, the resulting hydrophilic nanoparticles exhibit large and size-dependent r(2) relaxivity values.

    Journal Title

    Langmuir

    Volume

    30

    Issue/Number

    21

    Publication Date

    1-1-2014

    Document Type

    Article

    Language

    English

    First Page

    6197

    Last Page

    6208

    WOS Identifier

    WOS:000336952800025

    ISSN

    0743-7463

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