Two-Photon Excited Fluorescence of a Conjugated Polyelectrolyte and Its Application in Cell Imaging

Authors

    Authors

    A. Parthasarathy; H. Y. Ahn; K. D. Belfield;K. S. Schanze

    Comments

    Authors: contact us about adding a copy of your work at [email protected]

    Abbreviated Journal Title

    ACS Appl. Mater. Interfaces

    Keywords

    two-photon absorption; conjugated polyelectrolytes; near-infrared; cell; imaging; OPTICAL NONLINEARITY; POLYMER; SPECTROSCOPY; ABSORPTION; LADDER; PHOTOPHYSICS; ETHYNYLENE); SHELL; STATE; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary

    Abstract

    The two-photon excited fluorescence of a conjugated polyelectrolyte (CPE), PPESO(3), was studied in methanol and in water. The photophysical and amplified quenching properties of the CPE observed under two-photon excitation were comparable to the results obtained under one-photon excited conditions. Two-photon fluorescence microscopy performed with PPESO(3)-coated silica nanoparticles in He La cells provided images with significantly improved resolution compared to one-photon microscopy, demonstrating the utility of the CPE as a fluorescent probe in two-photon fluorescence cell imaging.

    Journal Title

    Acs Applied Materials & Interfaces

    Volume

    2

    Issue/Number

    10

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    2744

    Last Page

    2748

    WOS Identifier

    WOS:000283463700014

    ISSN

    1944-8244

    Share

    COinS
     

    Accessibility Statement

    This item was created or digitized prior to April 24, 2027, or is a reproduction of legacy media created before that date. It is preserved in its original, unmodified state specifically for research, reference, or historical recordkeeping. In accordance with the ADA Title II Final Rule, the University Libraries provides accessible versions of archival materials upon request. To request an accommodation for this item, please submit an accessibility request form.