Two-Photon Excited Fluorescence of a Conjugated Polyelectrolyte and Its Application in Cell Imaging

    Authors

    A. Parthasarathy; H. Y. Ahn; K. D. Belfield;K. S. Schanze

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    ACS Appl. Mater. Interfaces

    Keywords

    two-photon absorption; conjugated polyelectrolytes; near-infrared; cell; imaging; OPTICAL NONLINEARITY; POLYMER; SPECTROSCOPY; ABSORPTION; LADDER; PHOTOPHYSICS; ETHYNYLENE); SHELL; STATE; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary

    Abstract

    The two-photon excited fluorescence of a conjugated polyelectrolyte (CPE), PPESO(3), was studied in methanol and in water. The photophysical and amplified quenching properties of the CPE observed under two-photon excitation were comparable to the results obtained under one-photon excited conditions. Two-photon fluorescence microscopy performed with PPESO(3)-coated silica nanoparticles in He La cells provided images with significantly improved resolution compared to one-photon microscopy, demonstrating the utility of the CPE as a fluorescent probe in two-photon fluorescence cell imaging.

    Journal Title

    Acs Applied Materials & Interfaces

    Volume

    2

    Issue/Number

    10

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    2744

    Last Page

    2748

    WOS Identifier

    WOS:000283463700014

    ISSN

    1944-8244

    Share

    COinS