Title

Two-Photon Excited Fluorescence of a Conjugated Polyelectrolyte and Its Application in Cell Imaging

Authors

Authors

A. Parthasarathy; H. Y. Ahn; K. D. Belfield;K. S. Schanze

Comments

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Abbreviated Journal Title

ACS Appl. Mater. Interfaces

Keywords

two-photon absorption; conjugated polyelectrolytes; near-infrared; cell; imaging; OPTICAL NONLINEARITY; POLYMER; SPECTROSCOPY; ABSORPTION; LADDER; PHOTOPHYSICS; ETHYNYLENE); SHELL; STATE; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary

Abstract

The two-photon excited fluorescence of a conjugated polyelectrolyte (CPE), PPESO(3), was studied in methanol and in water. The photophysical and amplified quenching properties of the CPE observed under two-photon excitation were comparable to the results obtained under one-photon excited conditions. Two-photon fluorescence microscopy performed with PPESO(3)-coated silica nanoparticles in He La cells provided images with significantly improved resolution compared to one-photon microscopy, demonstrating the utility of the CPE as a fluorescent probe in two-photon fluorescence cell imaging.

Journal Title

Acs Applied Materials & Interfaces

Volume

2

Issue/Number

10

Publication Date

1-1-2010

Document Type

Article

Language

English

First Page

2744

Last Page

2748

WOS Identifier

WOS:000283463700014

ISSN

1944-8244

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