Title
High-Fidelity Hydrophilic Probe for Two-Photon Fluorescence Lysosomal Imaging
Abbreviated Journal Title
J. Am. Chem. Soc.
Keywords
MICROSCOPY; DISEASE; COMPLEX; Chemistry, Multidisciplinary
Abstract
The synthesis and characterization of a novel two-photon-absorbing fluorene derivative, LT1, selective for the lysosomes of HCT 116 cancer cells, is reported. Linear and nonlinear photophysical and photochemical properties of the probe were investigated to evaluate the potential of the probe for two-photon fluorescence microscopy (2PFM) lysosomal imaging. The cytotoxicity of the probe was investigated to evaluate the potential of using this probe for live two-photon fluorescence biological imaging applications. Colocalization studies of the probe with commercial Lysotracker Red in HCT 116 cells demonstrated the specific localization of the probe in the lysosomes with an extremely high colocalization coefficient (0.96). A figure of merit was introduced to allow comparison between probes. LT1 has a number of properties that far exceed those of commercial lysotracker probes, including higher two-photon absorption cross sections, good fluorescence quantum yield, and, importantly, high photostability, all resulting in a superior figure of merit. 2PFM was used to demonstrate lysosomal tracking with LT1.
Journal Title
Journal of the American Chemical Society
Volume
132
Issue/Number
35
Publication Date
1-1-2010
Document Type
Article
DOI Link
Language
English
First Page
12237
Last Page
12239
WOS Identifier
ISSN
0002-7863
Recommended Citation
"High-Fidelity Hydrophilic Probe for Two-Photon Fluorescence Lysosomal Imaging" (2010). Faculty Bibliography 2010s. 922.
https://stars.library.ucf.edu/facultybib2010/922
Comments
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