Title

High-Fidelity Hydrophilic Probe for Two-Photon Fluorescence Lysosomal Imaging

Authors

Authors

X. H. Wang; D. M. Nguyen; C. O. Yanez; L. Rodriguez; H. Y. Ahn; M. V. Bonder;K. D. Belfield

Comments

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Abbreviated Journal Title

J. Am. Chem. Soc.

Keywords

MICROSCOPY; DISEASE; COMPLEX; Chemistry, Multidisciplinary

Abstract

The synthesis and characterization of a novel two-photon-absorbing fluorene derivative, LT1, selective for the lysosomes of HCT 116 cancer cells, is reported. Linear and nonlinear photophysical and photochemical properties of the probe were investigated to evaluate the potential of the probe for two-photon fluorescence microscopy (2PFM) lysosomal imaging. The cytotoxicity of the probe was investigated to evaluate the potential of using this probe for live two-photon fluorescence biological imaging applications. Colocalization studies of the probe with commercial Lysotracker Red in HCT 116 cells demonstrated the specific localization of the probe in the lysosomes with an extremely high colocalization coefficient (0.96). A figure of merit was introduced to allow comparison between probes. LT1 has a number of properties that far exceed those of commercial lysotracker probes, including higher two-photon absorption cross sections, good fluorescence quantum yield, and, importantly, high photostability, all resulting in a superior figure of merit. 2PFM was used to demonstrate lysosomal tracking with LT1.

Journal Title

Journal of the American Chemical Society

Volume

132

Issue/Number

35

Publication Date

1-1-2010

Document Type

Article

Language

English

First Page

12237

Last Page

12239

WOS Identifier

WOS:000281460100031

ISSN

0002-7863

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