High-Fidelity Hydrophilic Probe for Two-Photon Fluorescence Lysosomal Imaging

    Authors

    X. H. Wang; D. M. Nguyen; C. O. Yanez; L. Rodriguez; H. Y. Ahn; M. V. Bonder;K. D. Belfield

    Comments

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    Abbreviated Journal Title

    J. Am. Chem. Soc.

    Keywords

    MICROSCOPY; DISEASE; COMPLEX; Chemistry, Multidisciplinary

    Abstract

    The synthesis and characterization of a novel two-photon-absorbing fluorene derivative, LT1, selective for the lysosomes of HCT 116 cancer cells, is reported. Linear and nonlinear photophysical and photochemical properties of the probe were investigated to evaluate the potential of the probe for two-photon fluorescence microscopy (2PFM) lysosomal imaging. The cytotoxicity of the probe was investigated to evaluate the potential of using this probe for live two-photon fluorescence biological imaging applications. Colocalization studies of the probe with commercial Lysotracker Red in HCT 116 cells demonstrated the specific localization of the probe in the lysosomes with an extremely high colocalization coefficient (0.96). A figure of merit was introduced to allow comparison between probes. LT1 has a number of properties that far exceed those of commercial lysotracker probes, including higher two-photon absorption cross sections, good fluorescence quantum yield, and, importantly, high photostability, all resulting in a superior figure of merit. 2PFM was used to demonstrate lysosomal tracking with LT1.

    Journal Title

    Journal of the American Chemical Society

    Volume

    132

    Issue/Number

    35

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    12237

    Last Page

    12239

    WOS Identifier

    WOS:000281460100031

    ISSN

    0002-7863

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