HBA1/2 mRNA Expression in UV Exposed Melanoma Cells

Authors

Victoria Sulaman, University of Central FloridaFollow
Victoria Woodcock, University of Central FloridaFollow

Streaming Media

Files

Course Code

PCB

Course Number

4943

Faculty/Instructor

Professor Nicole Verity

Faculty/Instructor Email

nicole.verity@ucf.edu

About the Author

I am Victoria Sulaman and my partner on this project is Victoria Woodcock. We are UCF PILOT students in the Burnett School of Biomedical Sciences. Thank you to Professor Nicole Verity, M.S. and Dr. Robert Borgon, PhD. for their guidance, support, and resources during this project.

You can view the video at https://ucf-my.sharepoint.com/:v:/g/personal/vi964698_ucf_edu/EQ2h0Hm3ffxApnyMoyaFHy0Bqx8c4iqdE1hQwFdR8mrJOg?nav=eyJyZWZlcnJhbEluZm8iOnsicmVmZXJyYWxBcHAiOiJTdHJlYW1XZWJBcHAiLCJyZWZlcnJhbFZpZXciOiJTaGFyZURpYWxvZy1MaW5rIiwicmVmZXJyYWxBcHBQbGF0Zm9ybSI6IldlYiIsInJlZmVycmFsTW9kZSI6InZpZXcifX0%3D&e=WyIBCH

Abstract, Summary, or Creative Statement

Progress has been made in finding ways to treat cancer. However, it remains a serious issue as many aspects of it are unclear. Specifically, melanoma is one of the most dangerous cancers because it is known to metastasize quickly. That said, our goal is to determine if there is an upregulation of HBA1/2 mRNA in melanoma cells as a result of oxidative stress from UV treatment. The foundation for this project was based on a paper where researchers found an upregulation of hemoglobin mRNA, specifically HBA1/2 mRNA, within keratinocytes when exposed to UVA radiation (Tahara et al., 2023). Their results suggested that hemoglobin acts like an antioxidant and can reduce oxidative stress, providing a mechanism to keep keratinocytes safe (Tahara et al., 2023). This semester, we conducted preliminary experiments to determine the optimal amount of time to expose our SK-MEL-28 (melanoma) cells to UV radiation, which was 90 minutes. This information will help us proceed with future experiments to figure out if there is a relationship between HBA1/2 mRNA upregulation and UV exposure in melanoma cells.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords

HBA1/2 mRNA and Melanoma; HBA1/2 mRNA and UV Radiation

Recommended Citation

Sulaman, Victoria and Woodcock, Victoria, "HBA1/2 mRNA Expression in UV Exposed Melanoma Cells" (2024). High Impact Practices Student Showcase Fall 2024. 68.
https://stars.library.ucf.edu/hip-2024fall/68