Osteoarthritis is a joint disease characterized by the breakdown of articular cartilage. The field of tissue engineering is interested in developing methods to produce biological alternatives to current orthopedic procedures. Lubricin is a molecule which is important in the proper lubrication of articular cartilage. It is a challenge in the field of tissue engineering to produce cartilage with sufficient lubricin expression. Developing a reporter cell for lubricin allowed for a more efficient investigation of the conditions which may influence its expression. By comparing "optimized" and traditional media solutions, it was determined that the use of a previously reported type II collagen optimized media would negatively affect the expression of lubricin. This information indicates the need to further evaluate the conditions which are conducive to producing cartilage with both sufficient types of type II collagen and lubricin.
Bachelor of Science (B.S.)
College of Medicine
Burnett School of Biomedical Sciences
Kennedy, Sean M., "Using a Lubricin Reporter Cell to Test Current vs. Optimized Media Compositions" (2021). Honors Undergraduate Theses. 891.