Factors released from TGF-B2 primed embryonic stem cells inhibit stress induced apoptosis in cardiomyoblasts

Abstract

Previous studies report oxidative stress induced apoptosis and necrosis occur following myocardial infarction. Effects of conditioned medium (CM) prepared from mouse embryonic stem (ES) cells on H2O2 induced apoptosis and necrosis in different cells types is under investigation. Additionally, effects of CM from ES cells primed with TGF-b2 on stress-induced apoptosis and necrosis in H9c2 cells have not been determined. In this study, H20i induced apoptosis was confirmed by trypan blue staining, terminal deoxynucleotide transferase dUTP-mediated nick-end labeling (TUNEL), and apoptotic enzyme labeled immunosorhent assay (ELISA) whereas necrosis was determined by LDH assay. Next, we generated CM from ES cells primed with and without TGF-b2 and determined their effects on H2O2 induced apoptosis and necrosis in H9c2 cells. Apoptosis and necrosis was significantly (P < 0.05) reduced with ES-CM compared with cell culture control. Next, our data showed TGF-B2 primed ES-CM further reduced cell death compared with ES-CM, suggesting increased amounts of cytoprotective released factors from mouse ES cells following TGF-B2 treatment. Furthermore, the treatment of H9c2 cells with TGF-b2 alone did not significantly (P < 0.05) reduce apoptotic cell death. In conclusion, we suggest that factors released from ES cells with and without TGF-B2 treatment contain anti-apoptotic and anti-necrotic factors that inhibit H2O2 induced cell death. Further studies are needed to determine potential additional benefits of the · released factors from TGF-B2 primed ES cells.

Notes

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Thesis Completion

2010

Semester

Spring

Advisor

Singla, Dinender

Degree

Bachelor of Science (B.S.)

College

Burnett School of Biomedical Sciences

Degree Program

Molecular Biology and Microbiology

Subjects

Biomedical Sciences -- Dissertations, Academic;Dissertations, Academic -- Biomedical Sciences

Format

Print

Identifier

DP0022534

Language

English

Access Status

Open Access

Length of Campus-only Access

None

Document Type

Honors in the Major Thesis

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