Pressure and temperature effects on bacteriorhodopsin light to dark adaptation studied by near-infrared raman spectroscopy

Abstract

Near-infrared Raman spectroscopy is employed to probe the light to dark adaptation in bacteriorhodopsin in the absence of undesired photoreactions. The C=C ethylenic stretching region in dark-adapted bacteriorhodopsin shows two resolved bands at 1526 and 1536 cm-1, which have been assigned to the all-trans and 13-cis isomers. Performing the first near-infrared Raman spectroscopic studies of bacteriorhodopsin at high pressure, we obtain direct spectroscopic evidence that the fraction of 13-cis isomers increases with increasing pressure. Assuming a two-state model, the equilibrium and kinetics of the light to dark-adaptation have been examined. Rate constants are presented as a function of temperature and pressure, and the conformational and activation volumes are determined.

Notes

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Thesis Completion

1994

Semester

Spring

Advisor

Schulte, Alfons

Degree

Bachelor of Science (B.S.)

College

College of Arts and Sciences

Degree Program

Physics

Subjects

Arts and Sciences -- Dissertations, Academic;Dissertations, Academic -- Arts and Sciences

Format

Print

Identifier

DP0020863

Language

English

Access Status

Open Access

Length of Campus-only Access

None

Document Type

Honors in the Major Thesis

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