A study of redox enzymes in Sulfolobus solfataricus and their potential use in bio-sensors
Abstract
In the characterization of many diseases, the tissues involved tend to have different chemical compositions when compared to those of normal tissues. For example, in certain types of cancer, metabolites or small peptides may be overproduced by the cancerous cells. If this difference can be measured, using bio-sensor technology, early on in disease progression and development, it may mean the difference between life and death for the patient suffering from the disease. Because of their substrate specificities and chemical recognition properties, Redox enzymes make ideal test subjects for studying the potential of enzymes for use in bio-sensing equipment. In this research, a target set of redox enzymes was cloned from the hyperthermophilic organism, Sulfolobus solfataricus. Many of the cloned enzymes were expressed and purified, so that biophysical characterizations, kinetic enzyme assays, and crystallization trials can be performed in the future.
Notes
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Thesis Completion
2005
Semester
Spring
Advisor
Self, William T.
Degree
Bachelor of Science (B.S.)
College
Burnett College of Biomedical Sciences
Degree Program
Molecular Biology and Microbiology
Subjects
Biomedical Sciences -- Dissertations, Academic; Dissertations, Academic -- Biomedical Sciences
Format
Identifier
DP0021982
Language
English
Access Status
Open Access
Length of Campus-only Access
None
Document Type
Honors in the Major Thesis
Recommended Citation
Small, Evan, "A study of redox enzymes in Sulfolobus solfataricus and their potential use in bio-sensors" (2005). HIM 1990-2015. 481.
https://stars.library.ucf.edu/honorstheses1990-2015/481