Keywords

Genomics; Conservation; Biology; DNA; Plant; Invertebrate

Abstract

Genomic resources are critical for the conservation of threatened species, providing valuable insights into genetic diversity, population structure, and adaptive potential. This thesis focuses on two ecologically interconnected species: the carnivorous plant pink sundew (Drosera capillaris) and its specialist herbivore the sundew plume moth (Buckleria parvulus). The sundew and plume moth face an uncertain future as their vital bog habitats continue to disappear. The sundew plays a crucial role in the life cycle of the plume moth, which relies on the sundew during its larval stages for survival. To aid in species detections, I first generated DNA barcodes for both the pink sundew (chloroplast matK gene) and the plume moth (mitochondrial COI gene) using Sanger sequencing. Next, using Illumina shotgun sequencing, I generated mitochondrial and chloroplast genome assemblies for each species. To generate a more complete chloroplast genome, I enriched chloroplasts with a chloroplast isolation kit then used Illumina sequencing to generate a second chloroplast genome. I then compared the novel and enriched chloroplast genomes using GetOrganelle and NOVOPlasty genome assemblers. Genomic resources from this study will provide taxonomic information and information on species specific adaptations and vulnerabilities. This information is useful for developing conservation strategies and ensuring the survival of these threatened species and preserving their unique ecological relationship.

Thesis Completion Year

2026

Thesis Completion Semester

Spring

Thesis Chair

Robert Fitak

College

College of Sciences

Department

Biology

Thesis Discipline

Biology

Language

English

Access Status

Open Access

Length of Campus Access

None

Campus Location

Orlando (Main) Campus

Download

Supplemental Files

Share

COinS
 

Accessibility Statement

This item was created or digitized prior to April 24, 2027, or is a reproduction of legacy media created before that date. It is preserved in its original, unmodified state specifically for research, reference, or historical recordkeeping. In accordance with the ADA Title II Final Rule, the University Libraries provides accessible versions of archival materials upon request. To request an accommodation for this item, please submit an accessibility request form.