Keywords
HBV; Split Deoxyribozyme; Fluorescence Assay; Nucleic Acid Detection; RNA Cleavage
Abstract
Rapid, accessible nucleic acid-based detection methods are needed for hepatitis B virus (HBV). A split deoxyribozyme assay was developed for sequence-specific fluorescence detection. The assay was evaluated using synthetic target and plasmid-derived material. The assay showed target-dependent fluorescence and low-concentration linear range with a LOD of 1.9 nM. The assay supports detection and semi-quantification of HBV genetic material as shown using plasmid-derived transcript samples from HBV genotypes B, C, and D. The selected target corresponded to nt 363–406 of the HBV genome and was chosen based on sequence alignment showing conservation across most genotypes. Genotype-specific detection remains a future application of this split probe design.
Thesis Completion Year
2026
Thesis Completion Semester
Spring
Thesis Chair
Gerasimova, Yulia
College
College of Sciences
Department
Chemistry
Thesis Discipline
Chemistry
Language
English
Access Status
Open Access
Length of Campus Access
None
STARS Citation
Zovko, Dalila, "Development of a Split Deoxyribozyme-Based Assay for Detecting Hepatitis B Virus" (2026). Honors Undergraduate Theses. 636.
https://stars.library.ucf.edu/hut2024/636
Included in
Biochemistry Commons, Immunology of Infectious Disease Commons, Molecular Biology Commons
Accessibility Statement
This item was created or digitized prior to April 24, 2027, or is a reproduction of legacy media created before that date. It is preserved in its original, unmodified state specifically for research, reference, or historical recordkeeping. In accordance with the ADA Title II Final Rule, the University Libraries provides accessible versions of archival materials upon request. To request an accommodation for this item, please submit an accessibility request form.