Abstract
The invention provides a method of co-culturing mammalian muscle cells and mammalian motoneurons. The method comprises preparing one or more carriers coated with a covalently bonded monolayer of trimethoxysilylpropyl diethylenetriamine (DETA); suspending isolated fetal mammalian skeletal muscle cells in serum-free medium according to medium composition 1; suspending isolated fetal mammalian spinal motoneurons in serum-free medium according to medium composition 1; plating the suspended muscle cells onto the one or more carriers at a predetermined density and allowing the muscle cells to attach; plating the suspended motoneurons at a predetermined density onto the one or more carriers and allowing the motoneurons to attach; covering the one or more carriers with a mixture of medium composition 1 and medium composition 2; and incubating the carriers covered in the media mixture.
Document Type
Patent
Patent Number
US 8,815,584
Application Serial Number
12/765,996
Issue Date
8-26-2014
Current Assignee
UCFRF
Assignee at Issuance
UCFRF
College
NanoScience Technology Center
Department
NanoScience Technology Center
Allowance Date
4-3-2014
Filing Date
4-23-2010
Assignee at Filing
UCFRF
Filing Type
Nonprovisional Application Record
Donated
no
Recommended Citation
Hickman, James and Das, Mainak, "Method of co-culturing mammalian muscle cells and motoneurons" (2014). UCF Patents. 346.
https://stars.library.ucf.edu/patents/346