Keywords
Coronary heart disease, Immunopathology, Milk, Anti-milk antibodies, Hemagglutination titer assay, IgM-mediated antibody response, Hemagglutination inhibition test, Complement fixation sensitivity
Abstract
Milk protein has been implicated as a factor in the development of atherosclerosis. Significantly higher titers of antibodies (P < 0.0002) toward milk antigens were observed in patients suffering from coronary heart disease as compared to age matched controls. These hemagglutination titers were not sex related but may have been related to age. Specificity of the antigen-antibody reaction was demonstrated by a hemagglutination inhibition test. The complement fixation test was evaluated and was less sensitive than the tanned hemaggIutination test. Treatment with 2-mercapto-ethanol resulted in reduced hemagglutination titers, indicating that significant antibody activity may be due to IgM. A special application of the Combs test detected specific antibodies on the surface of tanned and coated RBC which did not otherwise produce detectable agglutination.
Graduation Date
1977
Advisor
Sweeney, Michael J.
Degree
Master of Science (M.S.)
College
College of Natural Sciences
Degree Program
Biology
Format
Pages
57 pages
Language
English
Rights
Written permission granted by copyright holder to the University of Central Florida Libraries to digitize and distribute for nonprofit, educational purposes.
Length of Campus-only Access
None
Access Status
Masters Thesis (Open Access)
Identifier
DP0003521
Subjects
Coronary heart disease; Immunopathology; Milk; Autoantibodies--Analysis; Milk proteins; Coronary heart disease--Etiology; Cardiovascular system--Diseases--Immunological aspects; Coronary heart disease--Research
STARS Citation
Spinos, Efstathios, "Antibodies to Milk Antigens in Human Coronary Heart Disease" (1977). Retrospective Theses and Dissertations. 380.
https://stars.library.ucf.edu/rtd/380
Contributor (Linked data)
Collection (Linked data)
Accessibility Status
Searchable text
Included in
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