Abstract
The effectiveness of IL-2 in the enhancement of immune responses in B6-AKR and normal B6 mice was investigated. In vivo treatment of chimeras with murine IL-2 partially restored PFC responses of their splenocytes to SRBC. This treatment suppressed splenocytes IL-2 production in chimeras. In vivo treatment of chimeras with rhIL-2 resulted in a slight enhancement of mitogenic responses to Con-A. This treatment had no significant effect on mitogenic responses to PHA. The rhIL-2 treatment did not result in suppression of IL-2 production by chimeric splenocytes. Recombinant human IL-2 enhanced mitogenic responses of normal B6 and chimeric mice to PHA in all age groups tested. However, the mitogenic response to Con-A was enhanced only in older mice (130 days post-transplant). The duration of rhIL-2 exposure time was critical for the enhancement of mitogenic responses of B6 splenocytes to PHA. The enhancement of thymocyte mitogenic responses to Con-A or PHA was also dependent on the length of incubation time with rhIL-2. However, the enhancement of Con-A mitogenesis was independent of the duration of exposure time to rhIL-2. Treatment of mitogen-stimulated splenocytes with 7D4 antibody greatly inhibited cell proliferation of several concentrations of mitogen tested. The 7D4 antibody significantly inhibited the response of thymocytes cultured with rhIL-2 to PHA or Con-A. Studies with 7D4 antibody provided direct evidence for the involvement of IL-2 in the proliferation of splenocytes to T-cell mitogens and the proliferation of thymocytes and co-stimulatory assays.
Notes
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Graduation Date
1987
Semester
Summer
Advisor
Longley, Ross E.
Degree
Master of Science (M.S.)
College
College of Arts and Sciences
Degree Program
Microbiology
Format
Pages
71 p.
Language
English
Rights
Public Domain
Length of Campus-only Access
None
Access Status
Masters Thesis (Open Access)
Identifier
DP0020545
STARS Citation
Sazesh, Sharareh, "Analysis of Immune Enhancing Effects of Interleukin-2" (1987). Retrospective Theses and Dissertations. 5042.
https://stars.library.ucf.edu/rtd/5042
Accessibility Status
Searchable text