The effectiveness of IL-2 in the enhancement of immune responses in B6-AKR and normal B6 mice was investigated. In vivo treatment of chimeras with murine IL-2 partially restored PFC responses of their splenocytes to SRBC. This treatment suppressed splenocytes IL-2 production in chimeras. In vivo treatment of chimeras with rhIL-2 resulted in a slight enhancement of mitogenic responses to Con-A. This treatment had no significant effect on mitogenic responses to PHA. The rhIL-2 treatment did not result in suppression of IL-2 production by chimeric splenocytes. Recombinant human IL-2 enhanced mitogenic responses of normal B6 and chimeric mice to PHA in all age groups tested. However, the mitogenic response to Con-A was enhanced only in older mice (130 days post-transplant). The duration of rhIL-2 exposure time was critical for the enhancement of mitogenic responses of B6 splenocytes to PHA. The enhancement of thymocyte mitogenic responses to Con-A or PHA was also dependent on the length of incubation time with rhIL-2. However, the enhancement of Con-A mitogenesis was independent of the duration of exposure time to rhIL-2. Treatment of mitogen-stimulated splenocytes with 7D4 antibody greatly inhibited cell proliferation of several concentrations of mitogen tested. The 7D4 antibody significantly inhibited the response of thymocytes cultured with rhIL-2 to PHA or Con-A. Studies with 7D4 antibody provided direct evidence for the involvement of IL-2 in the proliferation of splenocytes to T-cell mitogens and the proliferation of thymocytes and co-stimulatory assays.
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Longley, Ross E.
Master of Science (M.S.)
College of Arts and Sciences
Length of Campus-only Access
Masters Thesis (Open Access)
Sazesh, Sharareh, "Analysis of Immune Enhancing Effects of Interleukin-2" (1987). Retrospective Theses and Dissertations. 5042.