Functional studies of murine T cells were performed to verify the role of T cells and adherent cells in the production of IL-2 and to clarify the role of the L3T4 receptor. Whole spleen cells were passed over nylon wool columns to obtain purified populations of cells for this purpose. It was found upon reconstitution of the T cells and adherent cells that a collaboration between the two was necessary for optimal IL-2 production. Their level of IL-2 production increased above the amount produced by the whole spleen cells. Antibody mediated cytotoxicity using L3T4 antisera identified T helper cells exclusively. Functional assays using concanavalin A to induce IL-2 production were performed on L3T4 depleted cells. The production of IL-2 was inhibited with L3T4 depletion to the same extent as Thy 1.2 depleted populations. This indicated that the L3T4 receptor was directly associated with the cells that specifically produce IL-2. The effects of concanavalin A stimulation on the number of L3T4 receptors found on T cells was also investigated. The results indicated that lectin stimulation had no effect on L3T4 receptor expression.
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Gennaro, Robert N.
Master of Science (M.S.)
College of Arts and Sciences
Length of Campus-only Access
Masters Thesis (Open Access)
Bass, Bonny, "T cell subset analysis in the C57BL/6J mouse" (1987). Retrospective Theses and Dissertations. 5051.
Contributor (Linked data)