SWGDAM developmental validation of a 19 locus Y-STR multiplex for forensic casework

Keywords

DNA -- Analysis; Forensic genetics; Y chromosome

Abstract

A comprehensive validation ofMultiplex I and Multiplex II, Y-STR systems developed at the National Center for Forensic Science, was completed using SWGDAM (Scientific Working Group on DNA Analysis Methods) standards. The combined Multiplex I and Multiplex II offer a robust co-amplification ofnineteen Y-STR markers including DYS393, DYS392, DYS391, DYS389I, DYS38911, Y-GATA-A7.2, DYS438, DYS385 a,b(MPI); DYS425, DYS388, DYS390, DYS439, DYS434, DYS437, YGATA-C.4, Y-GATA-A7.1, Y-GATA-H.4, and DYS19 (MPII). A useful characteristic ofthese multiplexes is the ability to detect male DNA in the presence ofoverwhelming female DNA. For both Multiplexes, when mixtures ofmale and female DNA were present, it was possible to detect the male component up to 1 :300. Additionally, using these Multiplexes, the number of males in a mixed stain can be determined. Sensitivity experiments show a full profile is obtainable at 150-200pg. Primate specificity was demonstrated by the lack ofcross reactivity with a variety of commonly encountered bacterial and animal species, with the exception ofa monomorphic canine product that was outside the range ofhuman alleles from any ofthe nineteen loci. Unsurprisingly cross reactivity was observed with a number ofmale and female non-human primates. Environmental and non-probative casework studies proved these Multiplexes to be robust under these conditions comparable with current autosomal methods. Measured reproducibility and precision using capillary electrophoresis was within expected norms. The validated Multiplexes demonstrate potential forensic use. In particular, these ii Multiplexes offer the capability to determine the number of male donors in a mixed stain and the ability to detect the male component in the presence ofan overabundance of female DNA. 111

Notes

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Graduation Date

2003

Advisor

Ballantyne, Jack

Degree

Master of Science (M.S.)

College

College of Arts and Sciences

Department

Chemistry

Format

PDF

Pages

120 p.

Language

English

Length of Campus-only Access

None

Access Status

Masters Thesis (Open Access)

Identifier

DP0028727

Subjects

Arts and Sciences -- Dissertations, Academic; Dissertations, Academic -- Arts and Sciences

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