Title
Microrna Let-7 Regulates 3T3-L1 Adipogenesis
Abstract
Differentiation of 3T3-L1 cells into adipocytes involves a highly orchestrated series of events including clonal expansion, growth arrest, and terminal differentiation. The mechanisms coordinating these different steps are not yet fully understood. Here we investigated whether microRNAs (miRNAs) play a role in this process. Microarray analysis was performed to detect miRNA expression during 3T3-L1 preadipocyte differentiation. Several miRNAs, including let-7, were up-regulated during 3T3-L1 adipogenesis. Ectopic introduction of let-7 into 3T3-L1 cells inhibited clonal expansion as well as terminal differentiation. The mRNA encoding high-mobility group AT-hook 2 (HMGA2), a transcription factor that regulates growth and proliferation in other contexts, was inversely correlated with let-7 levels during 3T3-L1 cell adipogenesis, and let-7 markedly reduced HMGA2 concentrations. Knockdown of HMGA2 inhibited 3T3-L1 differentiation. These results suggest that let-7 plays an important role in adipocyte differentiationandthat itdoesso in partbytargetingHMGA2,thereby regulating the transition from clonal expansion to terminal differentiation. Copyright © 2009 by The Endocrine Society.
Publication Date
6-1-2009
Publication Title
Molecular Endocrinology
Volume
23
Issue
6
Number of Pages
925-931
Document Type
Article
Personal Identifier
scopus
DOI Link
https://doi.org/10.1210/me.2008-0298
Copyright Status
Unknown
Socpus ID
66449092619 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/66449092619
STARS Citation
Sun, Tingwan; Fu, Mingui; Bookout, Angie L.; Kliewer, Steven A.; and Mangelsdorf, David J., "Microrna Let-7 Regulates 3T3-L1 Adipogenesis" (2009). Scopus Export 2000s. 11848.
https://stars.library.ucf.edu/scopus2000/11848