Title

Chloroplast-Derived Vaccine Antigens And Other Therapeutic Proteins

Keywords

Chloroplast genetic engineering; Oral delivery; Plant made biopharmaceuticals

Abstract

The chloroplast genetic engineering offers a number of unique advantages including high level of transgene expression, multi-gene expression in single transformation event and transgene containment due to maternal inheritance. Hyper-expression of vaccine antigens or therapeutic proteins in transgenic chloroplasts (leaves) or chromoplasts (fruits/roots) facilitates efficient oral delivery. Ability of chloroplasts to correctly fold human blood proteins with proper disulfide bridges (human serum albumin or interferons) or presence of chaperones in chloroplasts to facilitate assembly of complex multi-subunit proteins or their prokaryotic nature to express native bacterial genes (up to 46.1% total leaf protein) are attractive features for therapeutic protein production. Purification of therapeutic proteins has been achieved using novel purification strategies that do not require expensive column chromatography. © 2004 Elsevier Ltd. All rights reserved.

Publication Date

3-7-2005

Publication Title

Vaccine

Volume

23

Issue

15 SPEC. ISS.

Number of Pages

1779-1783

Document Type

Article; Proceedings Paper

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.vaccine.2004.11.004

Socpus ID

14744286603 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/14744286603

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