Title

Callus Induction And Regeneration In Spirodela And Lemna

Keywords

Duckweed; Galactose; Sorbitol; Tissue culture

Abstract

The development of tissue culture systems in duckweeds has, to date, been limited to species of the genus Lemna. We report here the establishment of an efficient tissue culture cycle (callus induction, callus growth and plant regeneration) for Spirodela oligorrhiza Hegelm SP, Spirodela punctata 8717 and Lemna gibba var. Hurfeish. Significant differences were found among the three duckweed species pertaining to carbohydrate and phytohormone requirements for callus induction, callus growth and frond regeneration. In vitro incubation with poorly assimilated carbohydrates such as galactose (S. oligorrhiza SP and L. gibba var. Hurfeish) and sorbitol (S. punctata 8717) as sole carbon source yielded high levels of callus induction on phytohormone-supplemented medium. Sorbitol is required for optimal callus growth of S. oligorrhiza SP and S. punctata 8717, while sucrose is required for callus growth of L. gibba var. Hurfeish. Sucrose either alone (S. oligorrhiza SP, L. gibba var. Hurfeish) or in addition to sorbitol (S. punctata 8717) is required for frond regeneration.

Publication Date

2-1-2004

Publication Title

Plant Cell Reports

Volume

22

Issue

7

Number of Pages

457-464

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1007/s00299-003-0724-4

Socpus ID

1242298970 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/1242298970

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