Title

Regulation Of Spine Development By Semaphorin3A Through Cyclin-Dependent Kinase 5 Phosphorylation Of Collapsin Response Mediator Protein 1

Keywords

Cdk5; Cerebral cortex; CRMP; PSD-95; Sema3A; Spine; Synapsin I

Abstract

Collapsin response mediator protein 1 (CRMP1) is one of the CRMP family members that mediates signal transduction of axonal guidance and neuronal migration. We show here evidence that CRMP1 is involved in semaphorin3A (Sema3A)-induced spine development in the cerebral cortex. In the cultured cortical neurons from crmp1+/- mice, Sema3A increased the density of clusters of synapsin I and postsynaptic density-95, but this increase was markedly attenuated in crmp1-/- mice. This attenuation was also seen in cyclin-dependent kinase 5 (cdk5)-/- neurons. Furthermore, the introduction of wild-type CRMP1 but not CRMP1-T509A/S522A, (Thr 509 and Ser 522 were replaced by Ala), a mutant that cannot be phosphorylated by Cdk5, into crmp1-/- neurons rescued the defect in Sema3A responsiveness. The Golgi-impregnation method showed that the crmp1-/- layer V cortical neurons showed a lower density of synaptic bouton-like structures and that this phenotype had genetic interaction with sema3A. These findings suggest that Sema3A-induced spine development is regulated by phosphorylation of CRMP1 by Cdk5. Copyright © 2007 Society for Neuroscience.

Publication Date

11-14-2007

Publication Title

Journal of Neuroscience

Volume

27

Issue

46

Number of Pages

12546-12554

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1523/JNEUROSCI.3463-07.2007

Socpus ID

36248979858 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/36248979858

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