Title

Noninvasive Tracking Of Cardiac Embryonic Stem Cells In Vivo Using Magnetic Resonance Imaging Techniques

Keywords

Cell transplantation; Embryonic stem cells; Heart; In vivo tracking; Mouse

Abstract

Despite rapid advances in the stem cell field, the ability to identify and track transplanted or migrating stem cells in vivo is limited. To overcome this limitation, we used magnetic resonance imaging (MRI) to detect and follow transplanted stem cells over a period of 28 days in mice using an established myocardial infarction model. Pluripotent mouse embryonic stem (mES) cells were expanded and induced to differentiate into beating cardiomyocytes in vitro. The cardiac-differentiated mES cells were then loaded with superparamagnetic fluorescent microspheres (1.63 μm in diameter) and transplanted into ischemic myocardium immediately following ligation and subsequent reperfusion of the left anterior descending coronary artery. To identify the transplanted stem cells in vivo, MRI was performed using a Varian Inova 4.7 Tesla scanner. Our results show that (a) the cardiac-differentiated mES were effectively loaded with superparamagnetic microspheres in vitro, (b) the microsphere-loaded mES cells continued to beat in culture prior to transplantation, (c) the transplanted mES cells were readily detected in the heart in vivo using noninvasive MRI techniques, (d) the transplanted stem cells were detected in ischemic myocardium for the entire 28-day duration of the study as confirmed by MRI and post-mortem histological analyses, and (e) concurrent functional MRI indicated typical loss of cardiac function, although significant amelioration of remodeling was noted after 28 days in hearts that received transplanted stem cells. These results demonstrate that it is feasible to simultaneously track transplanted stem cells and monitor cardiac function in vivo over an extended period using noninvasive MRI techniques. ©AlphaMed Press.

Publication Date

11-1-2007

Publication Title

Stem Cells

Volume

25

Issue

11

Number of Pages

2936-2944

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1634/stemcells.2007-0216

Socpus ID

36248931028 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/36248931028

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