Title

Differentiation Of Skeletal Muscle And Integration Of Myotubes With Silicon Microstructures Using Serum-Free Medium And A Synthetic Silane Substrate

Abstract

This protocol describes a cell culture model to study the differentiation of fetal rat skeletal muscle cells. The model uses serum-free medium, a nonbiological substrate W-1[3(trimethoxysilyl)propyl] diethylenetriamine (DETA) and fabricated microcantilevers to promote the differentiation of dissociated rat myocytes into robust myotubes. In this protocol, we also describe how to characterize the myotubes on the basis of morphology, immunocytochemistry and electrophysiology. Here, four major techniques are employed: fabrication of cantilevers, surface modification of the glass and cantilever substrates with a DETA SAM, a serum-free medium and refined culture techniques. This culture system has potential applications in biocompatibility studies, bioartificial muscle engineering, skeletal muscle differentiation studies and for better understanding of myopathies and neuromuscular disorders. © 2007 Nature Publishing Group.

Publication Date

6-28-2007

Publication Title

Nature Protocols

Volume

2

Issue

7

Number of Pages

1795-1801

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1038/nprot.2007.229

Socpus ID

34548589201 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/34548589201

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