Title

Surface-Derivatized Nanoceria With Human Carbonic Anhydrase Ii Inhibitors And Fluorophores: A Potential Drug Delivery Device

Abstract

Human carbonic anhydrase (hCAII) is a metalloenzyme that catalyzes the reversible hydration of carbon dioxide to bicarbonate and is associated with glaucoma (a major cause of blindness). The present study focuses on the use of cerium oxide nanoparticles (nanoceria) as a potential delivery device for hCAII inhibitors. Carboxybenzenesulfonamide, an inhibitor of the hCAII enzyme, was attached to nanoceria particles using epichlorohydrin as an intermediate linkage. Along with the CA inhibitor, a fluorophore (carboxyfluorescein) was also attached on the nanoparticles to enable the tracking of the nanoparticles in vitro as well as in vivo. X-ray photoelectron spectroscopic studies carried out at each reaction step confirmed the successful derivatization of the nanoceria particles. The attachment of carboxyfluorescein was also confirmed by confocal fluorescence microscopy. Preliminary studies suggest that carboxybenzenesulfonamide-functionalized nanoceria retains its inhibitory potency for hCAII. © 2007 American Chemical Society.

Publication Date

6-21-2007

Publication Title

Journal of Physical Chemistry C

Volume

111

Issue

24

Number of Pages

8437-8442

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/jp067666l

Socpus ID

34547266253 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/34547266253

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