Title
Protein Adsorption And Cellular Uptake Of Cerium Oxide Nanoparticles As A Function Of Zeta Potential
Keywords
Cellular uptake; Cerium oxide nanoparticles; Electrostatic interactions; Protein adsorption; Zeta potential
Abstract
The surface chemistry of biomaterials can have a significant impact on their performance in biological applications. Our recent work suggests that cerium oxide nanoparticles are potent antioxidants in cell culture models and we have evaluated several therapeutic applications of these nanoparticles in different biological systems. Knowledge of protein adsorption and cellular uptake will be very useful in improving the beneficial effects of cerium oxide nanoparticles in biology. In the present study, we determined the effect of zeta potential of cerium oxide nanoparticles on adsorption of bovine serum albumin (BSA) and cellular uptake in adenocarcinoma lung cells (A549). The zeta potential of the nanoparticles was varied by dispersing them in various acidic and basic pH solutions. UV-visible spectroscopy and inductively coupled plasma mass spectrometry (ICP-MS) were used for the protein adsorption and cellular uptake studies, respectively. Nanoceria samples having positive zeta potential were found to adsorb more BSA while the samples with negative zeta potential showed little or no protein adsorption. The cellular uptake studies showed preferential uptake for the negatively charged nanoparticles. These results demonstrate that electrostatic interactions can play an important factor in protein adsorption and cellular uptake of nanoparticles.
Publication Date
11-1-2007
Publication Title
Biomaterials
Volume
28
Issue
31
Number of Pages
4600-4607
Document Type
Article
Personal Identifier
scopus
DOI Link
https://doi.org/10.1016/j.biomaterials.2007.07.029
Copyright Status
Unknown
Socpus ID
34547903944 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/34547903944
STARS Citation
Patil, Swanand; Sandberg, Amanda; Heckert, Eric; Self, William; and Seal, Sudipta, "Protein Adsorption And Cellular Uptake Of Cerium Oxide Nanoparticles As A Function Of Zeta Potential" (2007). Scopus Export 2000s. 6310.
https://stars.library.ucf.edu/scopus2000/6310