Title

Combined Phase-Sensitive Acoustic Microscopy And Confocal Laser Scanning Microscopy

Keywords

Cells; Confocal laser scanning microscopy; Phase-sensitive acoustic microscopy; Polymer blend

Abstract

Combined phase-sensitive acoustic microscopy (PSAM) at 1.2 GHz and confocal laser scanning microscopy (CLSM) in reflection and fluorescence has been implemented and applied to polymer blend films and fluorescently labeled fibroblasts and neuronal cells in order to explore the prospects and the various contrast mechanisms of this powerful technique. Topographic contrast is available for appropriate samples from CLSM in reflection and, with significantly higher precision, from the acoustic phase images. Material contrast can be gained from acoustic amplitude V(z) graphs. In the case of the biological cells investigated, the optical and acoustic images are very different and exhibit different features of the samples. © 2006 Elsevier B.V. All rights reserved.

Publication Date

12-22-2006

Publication Title

Ultrasonics

Volume

44

Issue

SUPPL.

Number of Pages

-

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.ultras.2006.05.030

Socpus ID

33845705930 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/33845705930

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