Title

Dna Demethylation Of Human Mesenchymal Stem Cells Has Increased Potential To Become Neuronal Cells

Keywords

5-aza-deoxycytidine; BDNF; hMSCs; TrkB

Abstract

The multipotent nature of human mesenchymal stem cells (hMSCs) is limited by their tissue-specific character. Epigenetic modifications of stem cell lineage are necessary to overcome this barrier. We demonstrated that treatment of hMSCs with DNA demethylating agent, 5-aza-deoxycytidine induced expression of Nestin, an intermediate filament protein, and also up regulated the expression of Brain-derived neurotrophic factor (BDNF) receptor, TrkB. When these cells were co-cultured with differentiated human neural stem cells, a sub-population of hMSCs differentiated into neural cells resembling neuronal morphology. BDNF induced the phosphorylation of extracellular signal-regulated kinases (ERKs) and cAMP response element binding (CREB) protein by MAP Kinase dependent manner in 5-aza-deoxycytidine treated hMSCs. BDNF also induced the expression of βIII-tubulin protein. We concluded that changing the DNA methylation status of genome and BDNF are important for neural differentiation of hMSCs. Our findings suggested that treatment with DNA demethylation agent, 5-aza-deoxycytidine increased the multipotency of hMSCs. © 2008 Nova Science Publishers, Inc.

Publication Date

11-14-2008

Publication Title

Journal of Stem Cells

Volume

3

Issue

3

Number of Pages

173-182

Document Type

Article

Personal Identifier

scopus

Socpus ID

77953674605 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/77953674605

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