Title

Gold Nanoparticle Bioconjugates For Biomolecular Detection

Keywords

Antibody; Cancer biomarker; Dynamic light scattering; Gold nanoparticles; Homogeneous immunoassay

Abstract

We recently reported the development of a highly sensitive homogeneous one-step immunoassay for cancer biomarker detection.1 We applied this technology for the detection and assay of free PSA (prostate specific antigen), a biomarker associated with prostate cancer. Two gold nanoparticle probes, one spherical gold nanoparticle with a diameter around 35 nm, and a gold nanorod with a dimension of 10 by 40 nm, are coupled with a pair of monocolonal antibody anti-PSA, respectively. Upon mixing the two nanoparticle probes with sample solutions, freePSA antigen in sample solution caused a nanoparticle aggregation due to antibody-antigen binding. By measuring the degree of nanoparticle aggregation using a dynamic light scattering technique, the antigen concentration in the sample was quantified.

Publication Date

10-1-2008

Publication Title

Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008

Volume

2

Number of Pages

256-258

Document Type

Article; Proceedings Paper

Personal Identifier

scopus

Socpus ID

52649138402 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/52649138402

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