Title

Nanoceria Extend Photoreceptor Cell Lifespan In Tubby Mice By Modulation Of Apoptosis/Survival Signaling Pathways

Keywords

Apoptosis; Inherited retinal degeneration; Nanoceria; Neuroprotection; Oxidative stress; Tubby mouse

Abstract

Cerium oxide nanoparticles, nanoceria, are inorganic antioxidants that have catalytic activities which mimic those of the neuroprotective enzymes superoxide dismutase and catalase. We have previously shown that nanoceria preserve retinal morphology and prevent loss of retinal function in a rat light damage model. In this study, the homozygous tubby mutant mouse, which exhibits inherited early progressive cochlear and retinal degeneration, was used as a model to test the ability of nanoceria to slow the progression of retinal degeneration. Tubby mice were injected systemically, intracardially, with 20. μl of 1 mM nanoceria in saline, at postnatal day 10 and subsequently at P20 and P30 whereas saline injected and uninjected wild type (or heterozygous tubby) served as injected and uninjected controls, respectively. Assays for retinal function, morphology and signaling pathway gene expression were performed on P34 mice. Our data demonstrate that nanoceria protect the retina by decreasing Reactive Oxygen Species (ROS), up-regulating the expression of neuroprotection-associated genes; down-regulating apoptosis signaling pathways and/or up-regulating survival signaling pathways to slow photoreceptor degeneration. These data suggest that nanoceria have significant potential as global agents for therapeutic treatment of inherited retinal degeneration and most types of ocular diseases. © 2011 Elsevier Inc.

Publication Date

6-1-2011

Publication Title

Neurobiology of Disease

Volume

42

Issue

3

Number of Pages

514-523

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.nbd.2011.03.004

Socpus ID

79954632189 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/79954632189

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