Title
Two-Photon Sted Spectral Determination For A New V-Shaped Organic Fluorescent Probe With Efficient Two-Photon Absorption
Keywords
absorption; fluorene derivatives; fluorescence; laser scanning fluorescence microscopy; two-photon stimulated emission depletion
Abstract
Two-photon stimulated emission depletion (STED) cross sections were determined over a broad spectral range for a novel two-photon absorbing organic molecule, representing the first such report. The synthesis, comprehensive linear photophysical, two-photon absorption (2PA), and stimulated emission properties of a new fluorene-based compound, (E)-2-{3-[2-(7-(diphenylamino)-9,9- diethyl-9H-fluoren-2-yl)vinyl]-5-methyl-4-oxocyclohexa-2,5-dienylidene} malononitrile (1), are presented. Linear spectral parameters, including excitation anisotropy and fluorescence lifetimes, were obtained over a broad range of organic solvents at room temperature. The degenerate two-photon absorption (2PA) spectrum of 1 was determined with a combination of the direct open-aperture Z-scan and relative two-photon-induced fluorescence methods using 1 kHz femtosecond excitation. The maximum value of the 2PA cross section ∼1700 GM was observed in the main, long wavelength, one-photon absorption band. One- and two-photon stimulated emission spectra of 1 were obtained over a broad spectral range using a femtosecond pump-probe technique, resulting in relatively high two-photon stimulated emission depletion cross sections (∼1200 GM). A potential application of 1 in bioimaging was demonstrated through one- and two-photon fluorescence microscopy images of HCT 116 cells incubated with micelle-encapsulated dye. One, two, let's go! One- and two-photon stimulated emission spectra of 1 (left) are obtained over a broad spectral region by a fluorescence quenching femtosecond pump-probe technique (right). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication Date
10-24-2011
Publication Title
ChemPhysChem
Volume
12
Issue
15
Number of Pages
2755-2762
Document Type
Article
Personal Identifier
scopus
DOI Link
https://doi.org/10.1002/cphc.201100456
Copyright Status
Unknown
Socpus ID
80054734432 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/80054734432
STARS Citation
Belfield, Kevin D.; Bondar, Mykhailo V.; Morales, Alma R.; Padilha, Lazaro A.; and Przhonska, Olga V., "Two-Photon Sted Spectral Determination For A New V-Shaped Organic Fluorescent Probe With Efficient Two-Photon Absorption" (2011). Scopus Export 2010-2014. 2992.
https://stars.library.ucf.edu/scopus2010/2992