Title

Bone Morphogenetic Protein 7 Polarizes Thp-1 Cells Into M2 Macrophages

Keywords

Atherosclerosis; IL-10; Macrophages; Monocytes

Abstract

It was hypothesized that monocyte treatment with bone morphogenetic protein 7 (BMP7) would significantly enhance monocyte polarization into M2 macrophages as well as increasing the levels of anti-inflammatory cytokines. In a cell culture system using monocytes (human acute monocytic leukemia cell line THP-1), we studied the effects of BMP7 on monocytes polarizing into M2 macrophages. The data demonstrate that THP-1 cells contain a BMP type II receptor (BMPR2), and that its activation is significantly (p < 0.05) increased following treatment with BMP7. Furthermore, there was an increase of M2 macrophages, BMPR2, and anti-inflammatory cytokines interleukin (IL)-10 and IL-1ra compared with the respective controls. Moreover, treatment with BMP7 caused a significant (p < 0.05) decrease in the levels of pro-inflammatory cytokines IL-6, tumour necrosis factor (TNF-α), and monocyte chemotactic protein-1 (MCP-1), compared with the controls. In conclusion, we suggest for the first time that BMP7 has a unique potential to polarize monocytes into M2 macrophages, required for tissue repair, which will have significant applications for the treatment of atherosclerosis.

Publication Date

7-1-2012

Publication Title

Canadian Journal of Physiology and Pharmacology

Volume

90

Issue

7

Number of Pages

947-951

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1139/Y2012-102

Socpus ID

84863651284 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84863651284

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