Title

A Facile Synthesis Of Plga Encapsulated Cerium Oxide Nanoparticles: Release Kinetics And Biological Activity

Keywords

Actin; Arp2/3 complex; Chlamydia trachomatis; Tarp

Abstract

Actin polymerization is required for . Chlamydia trachomatis entry into nonphagocytic host cells. Host and chlamydial actin nucleators are essential for internalization of chlamydiae by eukaryotic cells. The host cell Arp2/3 complex and the chlamydial translocated actin recruiting phosphoprotein (Tarp) are both required for entry. Tarp and the Arp2/3 complex exhibit unique actin polymerization kinetics individually, but the molecular details of how these two actin nucleators cooperate to promote bacterial entry is not understood. In this study we provide biochemical evidence that the two actin nucleators act synergistically by co-opting the unique attributes of each to enhance the dynamics of actin filament formation. This process is independent of Tarp phosphorylation. We further demonstrate that Tarp colocalization with actin filaments is independent of the Tarp phosphorylation domain. The results are consistent with a model in which chlamydial and host cell actin nucleators cooperate to increase the rate of actin filament formation. © 2012 Elsevier Inc.

Publication Date

4-21-2012

Publication Title

Nanoscale

Volume

4

Issue

4

Number of Pages

2597-2605

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1039/c2nr12131j

Socpus ID

84859315929 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84859315929

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