Title

Pkcδ Mediates Paraquat-Induced Nox1 Expression In Dopaminergic Neurons

Keywords

NADPH oxidase; Paraquat; Parkinson Disease; Protein Kinase C delta

Abstract

Our previous works have shown that the (NADPH) oxidase (Nox) enzyme, in particular Nox1, plays an important role in oxidative stress and subsequent dopaminergic cell death elicited by paraquat (PQ). In non-neuronal and glial cells, protein kinase C δ (PKCδ) shows the ability to regulate the activity of the Nox system. Herein we aimed to investigate if also in dopaminergic neurons exposed to PQ, PKCδ can regulate Nox1 expression. The chemical inhibitor, rottlerin, and short interference RNA (siRNA) were used to inhibit or selectively knockdown PKCδ, respectively. The studies were performed using the immortalized rat mesencephalic dopaminergic cell line (N27 cells) exposed to PQ, after pre-incubation with rottlerin or transfected with PKCδ-siRNA. We observed that inhibition or knockdown of PKCδ significantly reduced PQ induced Nox1 transcript and protein levels, ROS generation and subsequent dopaminergic cell death. The results suggest that PKCδ plays a role in the regulation of Nox1-mediated oxidative stress elicited by PQ and could have a role in the pathogenesis of Parkinson's disease. © 2013 Elsevier Inc.

Publication Date

8-2-2013

Publication Title

Biochemical and Biophysical Research Communications

Volume

437

Issue

3

Number of Pages

380-385

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.bbrc.2013.06.085

Socpus ID

84880964990 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84880964990

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