Title

Detection Of Bacterial 16S Rrna Using A Molecular Beacon-Based X Sensor

Keywords

16S rRNA; DNA crossover; Hybridization probes; Molecular beacon probe; Nucleic acid detection

Abstract

We demonstrate how a long structurally constrained RNA can be analyzed in homogeneous solution at ambient temperatures with high specificity using a sophisticated biosensor. The sensor consists of a molecular beacon probe as a signal reporter and two DNA adaptor strands, which have fragments complementary to the reporter and to the analyzed RNA. One adaptor strand uses its long RNA-binding arm to unwind the RNA secondary structure. Second adaptor strand with a short RNA-binding arm hybridizes only to a completely complementary site, thus providing high recognition specificity. Overall the three-component sensor and the target RNA form a four-stranded DNA crossover (X) structure. Using this sensor, Escherichia coli16. S rRNA was detected in real time with the detection limit of ~0.17. nM. The high specificity of the analysis was proven by differentiating Bacillus subtilis from E. coli 16. S rRNA sequences. The sensor responds to the presence of the analyte within seconds. © 2012 Elsevier B.V.

Publication Date

3-15-2013

Publication Title

Biosensors and Bioelectronics

Volume

41

Issue

1

Number of Pages

386-390

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.bios.2012.08.058

Socpus ID

84870790859 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84870790859

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