Crispr-Sirius: Rna Scaffolds For Signal Amplification In Genome Imaging

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins to enhance brightness for live cell imaging of genomic loci. However, many of these modifications result in guide RNA instability and thus produce lower genome-labeling efficiency than anticipated. Here we introduce CRISPR-Sirius, based on octet arrays of aptamers conferring both enhanced guide RNA stability and brightness, and provide initial biological applications of this platform.

Publication Date

11-1-2018

Publication Title

Nature Methods

Volume

15

Issue

11

Number of Pages

928-931

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1038/s41592-018-0174-0

Socpus ID

85055818450 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85055818450

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